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10× rt reaction buffer  (DBI Bioscience)

 
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    DBI Bioscience 10× rt reaction buffer
    10× Rt Reaction Buffer, supplied by DBI Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10%C3%97+rt+reaction+buffer/10%C3%97+rt+reaction+buffer/pm33151695-73-9-12
    Average 90 stars, based on 1 article reviews
    10× rt reaction buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Virus:

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μl dNTP mixture, 2 μl 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μl of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term <i>in vivo</i> irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μL dNTP mixture, 2 μL 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μL of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: iTRAQ-Based Proteomic Profiling of Potential Biomarkers in Rat Serum for Uranium Tailing Suspension Intratracheal Instillation.
    Article Snippet: Protection against low-dose ionizing radiation is of great significance.. Uranium tailings are formed as a byproduct of uranium mining and a potential risk to organisms.. In this study, we identified potential biomarkers associated with exposure to low-dose radiation from uranium tailings.

    Reverse Transcription:

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μl dNTP mixture, 2 μl 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μl of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term <i>in vivo</i> irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μL dNTP mixture, 2 μL 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μL of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: iTRAQ-Based Proteomic Profiling of Potential Biomarkers in Rat Serum for Uranium Tailing Suspension Intratracheal Instillation.
    Article Snippet: Protection against low-dose ionizing radiation is of great significance.. Uranium tailings are formed as a byproduct of uranium mining and a potential risk to organisms.. In this study, we identified potential biomarkers associated with exposure to low-dose radiation from uranium tailings.

    cDNA Synthesis:

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μl dNTP mixture, 2 μl 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μl of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term <i>in vivo</i> irradiation by means of uranium tailings
    Article Snippet: .. Subsequently, 4 μL dNTP mixture, 2 μL 10× RT reaction buffer (DBI Bioscience, Ludwigshafen, Germany), 200 units of Moloney murine leukemia virus reverse transcriptase, and 1 μL of RNase inhibitor were added and first strand cDNA synthesis then performed by incubating the mixture at 37°C for 1 h and then at 95°C for 5 min, followed by cooling on ice. ..

    Article Title: iTRAQ-Based Proteomic Profiling of Potential Biomarkers in Rat Serum for Uranium Tailing Suspension Intratracheal Instillation.
    Article Snippet: Protection against low-dose ionizing radiation is of great significance.. Uranium tailings are formed as a byproduct of uranium mining and a potential risk to organisms.. In this study, we identified potential biomarkers associated with exposure to low-dose radiation from uranium tailings.



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